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Ribolli,Josiane; Scaranto,Bianca Maria; Shibatta,Oscar Akio; Bombardelli,Robie Allan; Zaniboni-Filho,Evoy. |
ABSTRACT DNA barcoding is a widely utilized molecular-based identification of species and taxonomic resolutions. Until recently, Rhamdia voulezi and Rhamdia branneri were considered species synonyms of Rhamdia quelen; however, morphological and cytogenetic analyses have suggested the validity of distinct species. Due to the absence of molecular taxonomy of R. voulezi and R. branneri, the objective of this study was to test its validity through traditional DNA barcoding and the GMYC (General Mixed Yule Coalescent) COI-based analyses in 19 specimens from the Iguaçu River Basin. In both methodologies, three MOTUs (Molecular Operational Taxonomic Units) were identified based on the estimated optimum threshold (OT = 0.77). The average inter-MOTU distance (NJ,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; COI; Jundiá; MOTU; Species complex. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100217 |
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Ribolli,Josiane; Miño,Carolina Isabel; Scaranto,Bianca Maria Soares; Reynalte-Tataje,David Augusto; Zaniboni Filho,Evoy. |
ABSTRACT Behavioral observations made on fish have revealed remarkably diverse reproductive strategies, including polygamy by both sexes. Still, to date, most Neotropical species remain unstudied as to whether the observed reproductive behavior in natural populations correlates with their genetic mating systems. Here, we investigated the genetic mating system of a wild population of Prochilodus lineatus settled in the Middle Uruguay River basin. By using sibship reconstruction and parental inference methods based on microsatellites’ genotypes, we inferred 45 females and 47 males as potential parents of the 87 larvae analyzed. We found evidence supporting polygamous mating in both sexes: while a high percentage of males (44.7%) fertilized the eggs of one... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic mating system; Microsatellites; Migratory fish; Parentage; Relatedness. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000200215 |
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Ribolli,Josiane; Zaniboni-Filho,Evoy. |
Supplementary stocking of fish in natural environments is a way to mitigate or compensate for the changes imposed on wild populations by river damming. Since little is known about the genetic composition of the supplementary stocks obtained by pooled-milt fertilization, the aim of this study was to determine the individual contributions of male jundiá Rhamdia quelen to offspring. Sperm from four males were mixed using equal volume of sperm from each of the males to fertilize eggs from only one female, kept in three blend with six males and three females. The proportions of larvae sired by the different males were quantified using five polymorphic DNA microsatellite loci. Analysis of these loci allowed paternal determination of 84% of the progeny, at a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Jundiá; Milt pool; Paternity; Genetic diversity. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400011 |
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