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DNA barcoding confirms the occurrence of Rhamdia branneri and Rhamdia voulezi (Siluriformes: Heptapteridae) in the Iguaçu River Basin Neotropical Ichthyology
Ribolli,Josiane; Scaranto,Bianca Maria; Shibatta,Oscar Akio; Bombardelli,Robie Allan; Zaniboni-Filho,Evoy.
ABSTRACT DNA barcoding is a widely utilized molecular-based identification of species and taxonomic resolutions. Until recently, Rhamdia voulezi and Rhamdia branneri were considered species synonyms of Rhamdia quelen; however, morphological and cytogenetic analyses have suggested the validity of distinct species. Due to the absence of molecular taxonomy of R. voulezi and R. branneri, the objective of this study was to test its validity through traditional DNA barcoding and the GMYC (General Mixed Yule Coalescent) COI-based analyses in 19 specimens from the Iguaçu River Basin. In both methodologies, three MOTUs (Molecular Operational Taxonomic Units) were identified based on the estimated optimum threshold (OT = 0.77). The average inter-MOTU distance (NJ,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaculture; COI; Jundiá; MOTU; Species complex.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100217
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Genetic characterization of the Neotropical catfish Pimelodus maculatus (Pimelodidae, Siluriformes) in the Upper Uruguay River Genet. Mol. Biol.
Ribolli,Josiane; Melo,Cláudio Manoel Rodrigues de; Zaniboni-Filho,Evoy.
Freshwater fish present unique challenges when one attempts to understand the factors that determine the structure of their populations. Habitat fragmentation is a leading cause of population decline that threatens ecosystems worldwide. In this study, we investigated the conservation status of genetic variability in the Neotropical catfish (Pimelodus maculatus). Specifically, we examined the structure and genetic diversity of this species in a region of the Upper Uruguay River fragmented by natural barriers and dams. There was no genetic structure among the four sites analyzed, indicating the existence of only one population group. A combination of environmental management and genetic monitoring should be used to minimize the impact of impoundment on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation genetics; Dams; Habitat fragmentation; Microsatellite.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500009
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Genetic evidence supports polygamous mating system in a wild population of Prochilodus lineatus (Characiformes: Prochilodontidae), a Neotropical shoal spawner fish Neotropical Ichthyology
Ribolli,Josiane; Miño,Carolina Isabel; Scaranto,Bianca Maria Soares; Reynalte-Tataje,David Augusto; Zaniboni Filho,Evoy.
ABSTRACT Behavioral observations made on fish have revealed remarkably diverse reproductive strategies, including polygamy by both sexes. Still, to date, most Neotropical species remain unstudied as to whether the observed reproductive behavior in natural populations correlates with their genetic mating systems. Here, we investigated the genetic mating system of a wild population of Prochilodus lineatus settled in the Middle Uruguay River basin. By using sibship reconstruction and parental inference methods based on microsatellites’ genotypes, we inferred 45 females and 47 males as potential parents of the 87 larvae analyzed. We found evidence supporting polygamous mating in both sexes: while a high percentage of males (44.7%) fertilized the eggs of one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic mating system; Microsatellites; Migratory fish; Parentage; Relatedness.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000200215
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Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen Neotropical Ichthyology
Ribolli,Josiane; Zaniboni-Filho,Evoy.
Supplementary stocking of fish in natural environments is a way to mitigate or compensate for the changes imposed on wild populations by river damming. Since little is known about the genetic composition of the supplementary stocks obtained by pooled-milt fertilization, the aim of this study was to determine the individual contributions of male jundiá Rhamdia quelen to offspring. Sperm from four males were mixed using equal volume of sperm from each of the males to fertilize eggs from only one female, kept in three blend with six males and three females. The proportions of larvae sired by the different males were quantified using five polymorphic DNA microsatellite loci. Analysis of these loci allowed paternal determination of 84% of the progeny, at a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jundiá; Milt pool; Paternity; Genetic diversity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400011
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Wide reproductive period of a long-distance migratory fish in a subtropical river, Brazil Neotropical Ichthyology
Zaniboni-Filho,Evoy; Ribolli,Josiane; Hermes-Silva,Samara; Nuñer,Alex P. O..
ABSTRACT Salminus brasiliensis is a potamodromous fish species that occurs in southern South American rivers. In spite of its ecological and economic relevance, information regarding the reproductive biology of S. brasiliensis is still scarce. This study used data from 18 years of continuous sampling in the Upper Uruguay River Basin, analyzing 718 adult fish (307 males, 243 females, 168 undefined) captured at different months of the year. The results showed that the reproductive timing for S. brasiliensis is wide in the Upper Uruguay River, with the occurrence of mature fish between the month of August and March and spawned individuals between July and May of the next year. These results were sustained by the increase of gonadal somatic relationship (GSR)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dourado; Long-term study; Population parameters; Salminus brasiliensis; Uruguay River.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100215
Registros recuperados: 5
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